Gateway instruction manual invitrogen

For more information on Gateway® MultiSite Technology, see the Gateway® Technology, MultiSite Gateway® and MultiSite Gateway® Pro manuals, available at www.doorway.ru Quality Control LR Clonase™ II Plus enzyme mix is functionally tested in a hour reaction to combine 4 fragments into one DEST vector, followed by a transformation assay. Gateway Cloning Technology - Instruction Manual Gateway Cloning Technology - Instruction Manual The Gateway® Technology is a universal cloning method based on the site-specific recombination properties of bacteriophage lambda Landy, The Gateway® Technology provides a rapid and highly efficient way to move DNA sequences into multiple vector. 2. Incubate for 60 min at 25°C. 3. Add 2 m l Proteinase K solution and incubate for 10 min at 37°C.: 4. Mix 1 m l of LR Reaction mix with 50 m l competent DH5 a cells (for the preparation see the appropriate protocol).: 5. Follow the protocol for the transformation of plasmid DNA to chemically competent E. coli cells (see protocol database).: 6. Plate 10 and m l of the final .

All Gateway ® product manuals are available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service Invitrogen™ Gateway™ cloning technology has been cited by life science researchers more than 2, times. Using BP Clonase Enzyme mix (Invitrogen), the genomic DNA fragments were cloned into the donor vector, pDONRzeo, according to the manufacturer's instruction manual After verifying the sequence integrity, the inserted DNA fragments were transferred to the binary vector, pMDC, encoding the bialaphos-resistance gene as a selectable marker. system (Invitrogen). Point mutants were made using the QuikChange site-directed mutagenesis kit (Stratagene). Gate-way LR reactions were performed as described in the Gateway cloning technology instruction manual (Invitrogen). Oligonu-cleotidesformutagenesis,PCR,andDNAsequencingreactions were obtained from Invitrogen. All plasmid constructs were.

converted Gateway® destination vector. 3. Introduce your expression clone into the appropriate host and express your recombinant protein. For more information on the Gateway® Technology, refer to the Gateway® Technology manual. This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service. We would like to show you a description here but the site won’t allow us. This Gateway Cloning instruction manual reviews: Recombination Reactions of the GATEWAY™ Cloning System The GATEWAY LR Cloning Reaction The GATEWAY BP Cloning Reaction Generating Entry Clones Designing Entry Clones for Protein Expression Location of Translation Start Sequences Reading Frame Examples of Protein Expression Constructs Destination Vectors GATEWAY Nomenclature Gateway.